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Aseptic Technique: Preparation of the Animal

Aseptic Technique in Rodent Surgery: Preparation of the Animal

Key points to note

  • Clip the fur from the surgical site after the animal has been anaesthetised.
  • Remove the clipped fur with a vacuum cleaner.
  • Perform this initial clipping in a separate area so that the operating area is not contaminated.
  • When clipping mice, particular care needs to be taken to avoid damaging the delicate skin.
  • Clean the skin using a suitable disinfectant, for example, Chlorhexidine, either in alcohol or water – but avoid using too much disinfectant solution, as this can increase the risk of the animal becoming hypothermic.
  • Alcohol alone is not suitable for disinfection of the skin.
  • In both rats and mice, ointment should be applied to the eyes to prevent drying during anaesthesia.
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